ISOLATION OF THE BACTERIA STRAINS HAVING THE CAPABLE OF PRODUCING INDOLE-3-ACETIC ACID (IAA) FROM PLANT SOIL NGOC LINH GINSENG IN KIM NOI, MU CANG CHAI, YEN BAI
Keywords:
biological characteristics, rhizosphere bacteria, auxin, Bacillus, Indole-3-acetic acid (IAA)Abstract
Indole-3-acetic acids are phytohormones belonging to the auxins. IAA has produced groups of microorganisms living in the rhizosphere bacteria root, such as Pseudomonas, Azospirillum, Azotobacter, Klebsiella, Enterobacter, Alcaligenes, Athobacter, Bacillus, and Serratia. From 4 samples of plant soil Ngoc Linh ginseng with an age of 1-2 years and isolated 14 bacteria strains capable of producing IAA, three strains with symbols YB26, YB38, and YBD2B7 are capable of producing IAA >20ug/ml. Study on biological characteristics of 03 strains with the results: good growth on carbon sources such as D-glucose and D mannitol; temperature 20-300C, pH 5 to 9, and 3 strains are able to produce extracellular enzymes such as amylase, cellulase, and protease. Classifying 02 strains based on 16S rRNA gene sequences, two strains, YB26 and YB38 have high similarity (>99%) with genes of strains of Bacillus. The fermentation fluid test of 02 strains increased the germination of seed vegetables by 4-9%.
References
[1]. Egorov N. X (Nguyễn Lân Dũng dịch), “Thực tập vi sinh vật học”, Nhà xuất bản Khoa học và Kỹ thuật, Hà Nội (1976).
[2]. Phan Công Du, Nguyễn Lê Quốc Hùng, Hoàng Thanh Tùng, Đỗ Mạnh Cường, Lê Xuân Thám, Dương Tấn Nhựt, “Nghiên cứu điều kiện nuôi trồng cây sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.) in vitro ở điều kiện nhà kính và tự nhiên tại Lâm Đồng”, Tạp chí Khoa học và công nghệ, 61 (12), 24- 31 (2019).
[3]. Trương Thị Chiên, Nguyễn Thị Thanh Mai, Nguyễn Xuân Cảnh, Nguyễn Ngọc Lan, Nguyễn Thị Hiền, Trần Bảo Trâm, “Phân lập và tuyển chọn chủng vi khuẩn có hoạt tính kháng Erwinia carotovora từ đất trồng sâm Ngọc Linh tại Quảng Nam”, Tạp chí Khoa học và công nghệ, 61 (12), 31- 35(2019).
[4]. Ali B., Sabri A. N., Ljung K., Hasnain S., “Quantification of indole-3-acetic acid from plant associated Bacillus spp. and their phytostimulatory effect on Vigna radiate (L.)”, World Journal of Microbiology and Biotechnology, 25(3), 519–526 (2009).
[5]. Brick J. M., Bostock R. M., Silverstone
S. E., “Rapid in situ assay for indole acetic acid production by bacteria immobilized on nitrocellulose membrane”, Applied and Environmental Microbiology, 57(2), 535–538 (1991).
[6]. Francis I., Holsters M., Vereecke D., “The Gram-positive side of plant–microbe interactions”, Environmental microbiology journal, 12(1), 1–12 (2010).
[7]. Han H. S., Lee K. D., “Plant Growth Promoting Rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of Lettuce under soil salinity”, Research Journal of Agriculture and Biological Sciences, 1(3), 210-215 (2005).
[8]. Kloepper J. W., Ryu C., Zhang S., “Induced systemic resistance and promotion of plant growth by Bacillus spp.”, Journal of Phytopathology, 94(11), 1259–1266 (2004).
[9]. Lo´pez B. J., Campos C. J., Herna´ndez C. E., Vela´squez B. C, Farias R. R, Macias R. L., Valencia C. E., “Bacillus megaterium rhizobacteria promote growth and alter root system architecture through an auxin and ethylene-independent signaling mechanism in Arabidopsis thaliana”, Molecular Plant- Microbe Interactions - APS Journals, 20(2), 207–217 (2007).
[10]. Mahalakshmi S., Reetha D., “Assessment of plant growth promoting activities of bacterial isolates from the rhizophere of tomato (Lycopersicon esculentum L.)”, Recent Research in Science and Technology, 1(1), 26- 29 (2009).
[11]. Perley J. W., Stowe B. B., “On the ability of Taphrina deformansto produce indole acetic acid from tryptophan by way of tryptamine”, Journal of Plant Physiology, 41(2), 234–237 (1966).
[12]. Phi Q. T., Oh S. H., Park Y. M., Park S. H., Ryu C. M., Ghim S. Y., “Isolation and characterization of transposon-insertional mutants from Paenibacillus polymyxa E681 altering the biosynthesis of indole-3-acetic acid”, Journal of Current Microbiology, 56(5), 524–530 (2008).
[13]. Spaepen S., Vanderleyden J., Remans R., “Indole-3-acetic acid in microbial and microorganism-plant signaling”, FEMS Microbiology Reviews, 31(4), 425–448 (2007).
[14]. Sambrook J., Russell D. W., Molecular clonning. A laboratory manual, 3rd ed, Cold spring harbor laboratory press, NewYork, (2001).
